Rosy bitterlings live in farm ponds (reservoirs) where freshwater mussels are abundant. Farm ponds are very important habitat for not only rosy bitterlings themselves also mussels and planktons. Freshwater mussels such as Dobugai play an important role in Rosy Bitterling reproduction.
Every female rosy bitterling has a unique pipe about the same length as its own body that is used for laying eggs on a (specific spot of mussels). Usually 2-3 eggs are laid at once and placed at the gill of the mussel. A male spawns into the gill cavity of the mussels right after a female lays eggs to ensure fertilization. Normally a female lays eggs repeatedly at 6-9 day intervals and about 10 times in a season.